Alpha 1000 Manual de usuario descargar pdf (Pagina 4)

Introduction

The Alpha-1000 is a single beam spectrophotometer, which is designed to meet the

needs of both students and instructors. Its digital display, easy operation, and

wavelength range of 400 nm to 1000 nm makes this unit ideal for

spectrophotometric experiments in the visible wavelength region of the

electromagnetic spectrum.

Working Principle

The spectrophotometer consists of five parts:

1) Light Source (Halogen lamp) to supply the light

2) A Monochromator to isolate the wavelength of interest and eliminate the

unwanted second order radiation

3) A Sample Compartment to accommodate the sample solution

4) A Detector to receive and convert the transmitted light to an electrical signal

5) A Digital Display to show absorbency and transmittance

Figure-1, illustrates the relationship between these parts.

Light Source Monochromator Sample Compartment Detector Digital Display

Figure-1 Block Diagram for the Spectrophotometer

In the spectrophotometer, light from the Halogen Lamp is focused on the entrance

slit of the Monochromator where the collimating mirror directs the beam onto the

grating. The grating disperses the light beam to produce the spectrum, a portion of

which is focused on the exit slit of the Monochromator by a collimating mirror. From

here, the beam is passed to a Sample Compartment through one of the filters,

which helps to eliminate unwanted second order radiation from the diffraction

grating. Upon leaving the Sample Compartment, the beam is passed to the silicon

photodiode Detector and causes the Detector to produce an electrical signal that is

shown on the Digital Display window.

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Alpha 1000 Manual de usuario Pagina 4